奧(ao)加伊妥珠(zhu)單(dan)抗（Besponsa；inotuzumabozogamicin）是(shi)輝瑞開發的(de)一款創新ADC，由(you)靶向(xiang)CD22的(de)單(dan)克隆抗體和細(xi)胞(bao)毒劑(ji)(ji)卡奇(qi)霉素兩部(bu)分組(zu)成。劑(ji)(ji)型為(wei)凍干(gan)粉，其制劑(ji)(ji)***中，蔗糖(tang)主要發揮凍干(gan)保護劑(ji)(ji)作用(yong)(yong)，氨丁三醇(chun)（TRIS）為(wei)pH緩沖(chong)劑(ji)(ji)。CD22抗原(yuan)在(zai)B細(xi)胞(bao)表面普遍(bian)存在(zai)，奧(ao)加伊妥珠(zhu)單(dan)抗能夠靶向(xiang)*細(xi)胞(bao)，并與之表面的(de)CD22抗原(yuan)結合。隨后(hou)，這(zhe)些ADC會(hui)(hui)被內吞入*細(xi)胞(bao)，卡奇(qi)霉素會(hui)(hui)進一步發揮它的(de)功效，造(zao)成*細(xi)胞(bao)的(de)死亡(wang)。2017年(nian)(nian)6月28日(ri)，BESPONSA獲歐盟批(pi)準(zhun)(zhun)上市。2017年(nian)(nian)8月17日(ri)，美國食品藥品監督管(guan)理(li)局(ju)（FDA）批(pi)準(zhun)(zhun)將BESPONSA用(yong)(yong)于***成人(ren)復發或難治性(xing)前體B細(xi)胞(bao)急性(xing)淋巴細(xi)胞(bao)白(bai)血病。值(zhi)得一提(ti)的(de)是(shi)，這(zhe)是(shi)***獲得FDA批(pi)準(zhun)(zhun)的(de)靶向(xiang)CD22的(de)抗體偶聯藥物。試(shi)劑(ji)(ji)級TRIS-HCl應用(yong)(yong)。山西輔料TRIS價格

【檢查】 ? pH10.0~11.510.0~11.510.0~11.5溶(rong)(rong)(rong)液(ye)的澄(cheng)清(qing)(qing)度與(yu)顏色(se)溶(rong)(rong)(rong)液(ye)應(ying)澄(cheng)清(qing)(qing)，幾乎無色(se)；如(ru)顯(xian)渾濁，與(yu)1號濁度標準液(ye)比(bi)(bi)較，不(bu)得(de)更濃。供(gong)試品(pin)溶(rong)(rong)(rong)液(ye)應(ying)澄(cheng)清(qing)(qing)，且(qie)無色(se)。--氯(lv)化物(wu)≦0.01%≦10ppm--有關物(wu)質照(zhao)薄(bo)層色(se)譜法試驗(yan)，供(gong)試品(pin)溶(rong)(rong)(rong)液(ye)（1)如(ru)顯(xian)雜(za)質斑(ban)(ban)點，其顏色(se)與(yu)對(dui)照(zhao)溶(rong)(rong)(rong)液(ye)所顯(xian)的主(zhu)斑(ban)(ban)點比(bi)(bi)較，不(bu)得(de)更深。照(zhao)薄(bo)層色(se)譜法試驗(yan)，供(gong)試品(pin)溶(rong)(rong)(rong)液(ye)（a)如(ru)顯(xian)雜(za)質斑(ban)(ban)點，其顏色(se)與(yu)對(dui)照(zhao)溶(rong)(rong)(rong)液(ye)（b）所顯(xian)的主(zhu)斑(ban)(ban)點比(bi)(bi)較，不(bu)得(de)更深。（≤1.0%）--干燥(zao)失重≦0.6%≦0.5%≦1.0%硫酸灰分(fen)--≦0.1%--熾(chi)灼(zhuo)殘渣≦0.1%--≦0.1%鐵鹽≦0.001%≦10ppm--鎳鹽≦0.0015%----重金屬≦10ppm----細菌內(nei)***供(gong)注射用：

緩(huan)(huan)沖(chong)(chong)溶(rong)液(ye)的(de)(de)用(yong)(yong)(yong)途在(zai)有(you)(you)機(ji)(ji)系(xi)統中，天然緩(huan)(huan)沖(chong)(chong)溶(rong)液(ye)可將pH值保持在(zai)恒定(ding)(ding)水(shui)平，從而可能發(fa)生(sheng)生(sheng)化反(fan)應而不會損害有(you)(you)機(ji)(ji)體(ti)(ti)。當(dang)生(sheng)物(wu)學家研究生(sheng)物(wu)學過程時，他(ta)們(men)必(bi)須保持相同(tong)的(de)(de)pH值。為此，他(ta)們(men)使用(yong)(yong)(yong)了準備好的(de)(de)緩(huan)(huan)沖(chong)(chong)溶(rong)液(ye)。緩(huan)(huan)沖(chong)(chong)溶(rong)液(ye)的(de)(de)產(chan)品和概念于1966年***被(bei)描述，我們(men)使用(yong)(yong)(yong)的(de)(de)緩(huan)(huan)沖(chong)(chong)與許多年前的(de)(de)緩(huan)(huan)沖(chong)(chong)液(ye)是(shi)相同(tong)的(de)(de)。為了有(you)(you)用(yong)(yong)(yong)，生(sheng)物(wu)緩(huan)(huan)沖(chong)(chong)液(ye)必(bi)須滿足多個標準。具體(ti)(ti)而言，它(ta)(ta)們(men)應該是(shi)水(shui)溶(rong)性的(de)(de)，但不溶(rong)于有(you)(you)機(ji)(ji)溶(rong)劑。它(ta)(ta)們(men)不應能夠(gou)通過細胞膜。另外，在(zai)使用(yong)(yong)(yong)它(ta)(ta)們(men)的(de)(de)所有(you)(you)實驗(yan)中，它(ta)(ta)們(men)必(bi)須是(shi)無毒(du)，惰性且穩定(ding)(ding)的(de)(de)。

詳情請(qing)見(jian)(jian)AVT官網，如有(you)(you)任(ren)何疑問歡(huan)迎給我(wo)們留言。量取下列溶液(ye)，置于(yu)lL燒杯中。1MTris-HClBuffer，100ml500mMEDTA20ml。向燒杯中加入約800ml的(de)去離子水(shui)，均(jun)勻混(hun)合(he)。將溶液(ye)定容至1L后(hou)，高溫(wen)(wen)高壓滅菌，室溫(wen)(wen)保存(cun)就(jiu)可以(yi)了(le)。介紹：三羥(qian)甲(jia)(jia)基氨(an)基甲(jia)(jia)烷(wan)是一種(zhong)有(you)(you)機(ji)化(hua)合(he)物，其分子式為(HOCH2)3CNH2。Tris被廣(guang)(guang)泛(fan)應用于(yu)生物化(hua)學(xue)(xue)和(he)分子生物學(xue)(xue)實(shi)(shi)驗(yan)中的(de)緩(huan)(huan)(huan)沖(chong)液(ye)的(de)制備(bei)。在生物化(hua)學(xue)(xue)實(shi)(shi)驗(yan)中常(chang)用的(de)TAE和(he)TBE緩(huan)(huan)(huan)沖(chong)液(ye)都(dou)需要(yao)用到Tris。Tris緩(huan)(huan)(huan)沖(chong)液(ye)不僅(jin)被廣(guang)(guang)用作(zuo)核酸(suan)和(he)蛋白質(zhi)的(de)溶劑(ji)，還有(you)(you)許多重要(yao)用途。詳情請(qing)見(jian)(jian)AVT官網，如有(you)(you)任(ren)何疑問歡(huan)迎給我(wo)們留言藥用級TRIS-HCl中美雙(shuang)報注意事項！

前述(shu)不(bu)同(tong)(tong)緩(huan)(huan)(huan)(huan)(huan)沖(chong)(chong)(chong)(chong)(chong)溶(rong)(rong)(rong)(rong)液(ye)(ye)(ye)(ye)雖然有(you)相(xiang)似(si)之(zhi)處，同(tong)(tong)時也存在(zai)差異，主要體(ti)現為(wei)以(yi)下幾(ji)點：（1）緩(huan)(huan)(huan)(huan)(huan)沖(chong)(chong)(chong)(chong)(chong)溶(rong)(rong)(rong)(rong)液(ye)(ye)(ye)(ye)應(ying)(ying)用(yong)的酸(suan)堿度環境有(you)差異：硼(peng)酸(suan)鹽緩(huan)(huan)(huan)(huan)(huan)沖(chong)(chong)(chong)(chong)(chong)溶(rong)(rong)(rong)(rong)液(ye)(ye)(ye)(ye)和(he)Tris-HCl緩(huan)(huan)(huan)(huan)(huan)沖(chong)(chong)(chong)(chong)(chong)溶(rong)(rong)(rong)(rong)液(ye)(ye)(ye)(ye)較(jiao)適(shi)合在(zai)堿性環境中使用(yong)，而磷酸(suan)鹽緩(huan)(huan)(huan)(huan)(huan)沖(chong)(chong)(chong)(chong)(chong)溶(rong)(rong)(rong)(rong)液(ye)(ye)(ye)(ye)和(he)HEPES緩(huan)(huan)(huan)(huan)(huan)沖(chong)(chong)(chong)(chong)(chong)溶(rong)(rong)(rong)(rong)液(ye)(ye)(ye)(ye)則多數情況下適(shi)宜運用(yong)在(zai)pH值為(wei)6.8~8.2左右(you)的弱酸(suan)性、弱堿性或(huo)接(jie)近中性的環境中；（2）緩(huan)(huan)(huan)(huan)(huan)沖(chong)(chong)(chong)(chong)(chong)溶(rong)(rong)(rong)(rong)液(ye)(ye)(ye)(ye)組(zu)成(cheng)有(you)差異：硼(peng)酸(suan)鹽緩(huan)(huan)(huan)(huan)(huan)沖(chong)(chong)(chong)(chong)(chong)溶(rong)(rong)(rong)(rong)液(ye)(ye)(ye)(ye)、Tris-HCl緩(huan)(huan)(huan)(huan)(huan)沖(chong)(chong)(chong)(chong)(chong)溶(rong)(rong)(rong)(rong)液(ye)(ye)(ye)(ye)及(ji)磷酸(suan)鹽緩(huan)(huan)(huan)(huan)(huan)沖(chong)(chong)(chong)(chong)(chong)溶(rong)(rong)(rong)(rong)液(ye)(ye)(ye)(ye)一(yi)(yi)般是由(you)兩(liang)種(zhong)物(wu)質配置而成(cheng)，而HEPES緩(huan)(huan)(huan)(huan)(huan)沖(chong)(chong)(chong)(chong)(chong)溶(rong)(rong)(rong)(rong)液(ye)(ye)(ye)(ye)則是由(you)一(yi)(yi)種(zhong)物(wu)質配制；（3）緩(huan)(huan)(huan)(huan)(huan)沖(chong)(chong)(chong)(chong)(chong)溶(rong)(rong)(rong)(rong)液(ye)(ye)(ye)(ye)發(fa)(fa)揮(hui)緩(huan)(huan)(huan)(huan)(huan)沖(chong)(chong)(chong)(chong)(chong)作用(yong)的原理有(you)一(yi)(yi)定(ding)差異：硼(peng)酸(suan)鹽緩(huan)(huan)(huan)(huan)(huan)沖(chong)(chong)(chong)(chong)(chong)溶(rong)(rong)(rong)(rong)液(ye)(ye)(ye)(ye)、Tris-HCl緩(huan)(huan)(huan)(huan)(huan)沖(chong)(chong)(chong)(chong)(chong)溶(rong)(rong)(rong)(rong)液(ye)(ye)(ye)(ye)及(ji)磷酸(suan)鹽緩(huan)(huan)(huan)(huan)(huan)沖(chong)(chong)(chong)(chong)(chong)溶(rong)(rong)(rong)(rong)液(ye)(ye)(ye)(ye)靠(kao)體(ti)系(xi)中緩(huan)(huan)(huan)(huan)(huan)沖(chong)(chong)(chong)(chong)(chong)對的電離或(huo)水解平(ping)衡移動來發(fa)(fa)揮(hui)緩(huan)(huan)(huan)(huan)(huan)沖(chong)(chong)(chong)(chong)(chong)作用(yong)，而HEPES緩(huan)(huan)(huan)(huan)(huan)沖(chong)(chong)(chong)(chong)(chong)溶(rong)(rong)(rong)(rong)液(ye)(ye)(ye)(ye)則是靠(kao)體(ti)系(xi)中平(ping)衡轉化(hua)關(guan)系(xi)來發(fa)(fa)揮(hui)緩(huan)(huan)(huan)(huan)(huan)沖(chong)(chong)(chong)(chong)(chong)作用(yong)；（4）緩(huan)(huan)(huan)(huan)(huan)沖(chong)(chong)(chong)(chong)(chong)溶(rong)(rong)(rong)(rong)液(ye)(ye)(ye)(ye)發(fa)(fa)揮(hui)作用(yong)的側重方面不(bu)同(tong)(tong)：如(ru)(ru)同(tong)(tong)是在(zai)生物(wu)藥(yao)(yao)相(xiang)關(guan)方面的應(ying)(ying)用(yong)，磷酸(suan)鹽緩(huan)(huan)(huan)(huan)(huan)沖(chong)(chong)(chong)(chong)(chong)溶(rong)(rong)(rong)(rong)液(ye)(ye)(ye)(ye)在(zai)固體(ti)藥(yao)(yao)物(wu)釋放研究(jiu)中應(ying)(ying)用(yong)較(jiao)多。而硼(peng)砂緩(huan)(huan)(huan)(huan)(huan)沖(chong)(chong)(chong)(chong)(chong)溶(rong)(rong)(rong)(rong)液(ye)(ye)(ye)(ye)多用(yong)于液(ye)(ye)(ye)(ye)體(ti)藥(yao)(yao)物(wu)如(ru)(ru)滴眼液(ye)(ye)(ye)(ye)中。Tris-HCl緩(huan)(huan)(huan)(huan)(huan)沖(chong)(chong)(chong)(chong)(chong)較(jiao)多用(yong)于蛋白(bai)質、DNA等生物(wu)大分(fen)子(zi)的溶(rong)(rong)(rong)(rong)液(ye)(ye)(ye)(ye)體(ti)系(xi)中。而HEPES更側重應(ying)(ying)用(yong)于脂質體(ti)、細胞水平(ping)的相(xiang)關(guan)生化(hua)實驗(yan)研究(jiu)中；藥(yao)(yao)用(yong)級TRIS-HCl已登(deng)記。海南現貨TRIS如(ru)(ru)何購買

AVT多(duo)級別TRIS現貨直銷。山西輔料TRIS價格

實際應(ying)用(yong)中各緩(huan)沖(chong)(chong)對組成(cheng)的(de)(de)(de)緩(huan)沖(chong)(chong)溶(rong)(rong)液(ye)(ye)(ye)都(dou)有(you)比較好(hao)的(de)(de)(de)緩(huan)沖(chong)(chong)范圍。在實際生產(chan)(chan)過程中，如果選擇(ze)的(de)(de)(de)緩(huan)沖(chong)(chong)溶(rong)(rong)液(ye)(ye)(ye)緩(huan)沖(chong)(chong)范圍與預期(qi)(qi)偏差較大，使用(yong)大量(liang)強酸/強堿或許可(ke)以強行將溶(rong)(rong)液(ye)(ye)(ye)pH調至預期(qi)(qi)值，但此(ci)時溶(rong)(rong)液(ye)(ye)(ye)體系的(de)(de)(de)緩(huan)沖(chong)(chong)容(rong)量(liang)已消耗殆盡，無法起到應(ying)有(you)的(de)(de)(de)緩(huan)沖(chong)(chong)保護(hu)效果，對制(zhi)劑產(chan)(chan)品后續(xu)的(de)(de)(de)加(jia)工及長期(qi)(qi)儲存穩定性造成(cheng)影(ying)響。因(yin)此(ci)，要用(yong)好(hao)緩(huan)沖(chong)(chong)溶(rong)(rong)液(ye)(ye)(ye)，需(xu)對不同的(de)(de)(de)緩(huan)沖(chong)(chong)溶(rong)(rong)液(ye)(ye)(ye)有(you)具體的(de)(de)(de)了解(jie)。以下介紹幾種(zhong)常見的(de)(de)(de)緩(huan)沖(chong)(chong)溶(rong)(rong)液(ye)(ye)(ye)及用(yong)途，為大家深入理解(jie)緩(huan)沖(chong)(chong)溶(rong)(rong)液(ye)(ye)(ye)提供(gong)參考。艾偉拓氨丁三醇山西輔(fu)料TRIS價格(ge)